Unexplained DNA Melting Behavior in a Genotyping Assay
نویسندگان
چکیده
منابع مشابه
Unexplained DNA melting behavior in a genotyping assay.
tion. We believe that this change will significantly increase the sensitivity for detecting myocardial necrosis (1, 3). In summary, we have shown that the upper reference limit for a healthy population is 0.16 g/L for TnI concentrations measured using the Bayer ACS:Centaur method. The difference between TnI values obtained for serum or heparin-plasma specimens is only just significant at the lo...
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BACKGROUND DNA hairpins have been used in molecular analysis of PCR products as self-probing amplicons. Either physical separation or fluorescent oligonucleotides with covalent modifications were previously necessary. METHODS We performed asymmetric PCR for 40-45 cycles in the presence of the saturating DNA dye, LCGreen Plus, with 1 primer including a 5' tail complementary to its extension pr...
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BACKGROUND High-resolution DNA melting is a closed-tube method for genotyping and variant scanning that depends on the thermal stability of PCR-generated products. Instruments vary in thermal precision, sample format, melting rates, acquisition, and software. Instrument genotyping accuracy has not been assessed. METHODS Each genotype of the single nucleotide variant (SNV) (c.3405-29A>T) of CP...
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Fluorescent nucleic acid detection in polymerase chain reaction (PCR) generally uses oligonucleotide probes labeled with covalently attached dyes. However, unlabeled oligonucleotides in the presence of saturating DNA dyes can also serve as hybridization probes. The DNA dye, LCGreen Plus, and a 3'-blocked unlabeled probe are added before amplification, and asymmetric PCR is performed at a 1:5 to...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2002
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/48.1.199